Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (1): 1-005.doi: 10.3969/j.issn.1004-616x.2002.01.001

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CONSTRUCTION OF DIFFERENTIALLY EXPRESSED cDNA LIBRARIES FROM DISSENMILATORYMALIGNANT TRANSFORMED HUMAN BRONCHIAL EPITHELIAL CELLS INDUCED BY ALPHAPARTICLE RADIATION

FAN Bao-xing;ZHANG Kai-tai; XIE Ling; XIANG Xiao-qiong; WU De-chang   

  1. Department of Molecular Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China
  • Received:2001-02-12 Revised:2001-06-26 Online:2002-01-30 Published:2002-01-30
  • Contact: FAN Bao-xing

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Abstract: Purpose : To construct differentially expressed cDNA libraries from different malignant transformed human bronchial epithelial cells induced by alpha-particle radiation. Methods : Suppression subtractive hybridization (SSH) . Results : Three differentially expressed cDNA libraries were constructed from different malignant transformed human bronchial epithelial cells. The number of clones is 416 in A subtraction library (The cDNAs of BEP2D cells as tester and R15Hp35 cells as driver) , 301 in B subtraction library ( The cDNAs of R15Hp20 cells as tester and R15Hp35 and BEP2D cells mixed together as driver. ) and 568 in C subtraction library (The cDNAs of R15Hp35 cells as tester and BEP2D cells as driver) . After 70 cDNAs were sequenced and analyzed , 61 cDNAs were found to be known genes , and 9 cDNAs were found to be novel ones. Conclusion : The cDNAs of three subtraction libraries may represent differentially expressed cDNA of different malignant transformed human bronchial epithelial cells induced by alpha-particle radiation.The data provide a basis for further investigation of the molecular mechanism of lung cancer induced by alpha-particle radiation.

Key words: Suppression subtractive hybridization, BEP2D cell, cell transformation

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